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Scaffolds for cell culture

N-heptyl-D-galactonamide, a small sugar-based amphiphile, gives biocompatible hydrogels. They have been used as scaffolds for neuron cell culture.

Neuro2A-GFP cell line grown inside a N-heptyl-D-galactonamide hydrogel (7 days) Cells are observed in fluorescence (green) Fibers are observed in reflexion (white/grey)
Neuro2A-GFP cell line observed in fluorescence (green) after 7 days Thin fibers stained with polylysine-FITC are observed in fluorescence (green) Large fibers are observed in reflexion mode (colorized in pink)

Publications

Neuro2A cell line
Neuro2A cell line labeled by immunofluorescence (green: Tuj1, blue: DAPI, red: Phalloidin, 7 days). The N-heptyl-D-galactonamide hydrogel fibers are observed in the background (grey).
Neuro2A cell line
Neuro2A cell line labeled by immunofluorescence (green: Tuj1, red: GFAP, blue: DAPI) after dissolution of the N-heptyl-D-galactonamide hydrogel (7 days)
Scaffold 3D detail
Confocal

Neurons network (left) and neurosphere (right) of adult Human Neural Stem Cells (hNSC) (7 days, green immunofluorescent marker : Tuj1), after dissolution of the N-heptyl-D-galactonamide hydrogel.

Glial cells from adult Human Neural Stem Cells (hNSC)  (7 days, green immunofluorescent marker : S100B), after dissolution of the N-heptyl-D-galactonamide hydrogel.