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Scaffolds for cell culture
N-heptyl-D-galactonamide, a small sugar-based amphiphile, gives biocompatible hydrogels. They have been used as scaffolds for neuron cell culture.
![](https://assemblingsugars.fr/wp-content/uploads/2020/05/Neuro2A-GFP.jpg)
![](https://assemblingsugars.fr/wp-content/uploads/2020/04/Composite-Image-Neuro2A-web-1024x1024.jpg)
Publications
- Chalard, A.; Vaysse, L.; Joseph, P.; Malaquin, L.; Souleille, S.; Lonetti, B.; Sol, J.-C.; Loubinoux, I.; Fitremann, J. Simple Synthetic Molecular Hydrogels from Self-Assembling Alkylgalactonamides as Scaffold for 3D Neuronal Cell Growth. ACS Appl. Mater. Interfaces 2018, 10 (20), 17004–17017.
![Neuro2A cell line](https://assemblingsugars.fr/wp-content/uploads/2020/06/image33.jpg)
![Neuro2A cell line](https://assemblingsugars.fr/wp-content/uploads/2020/06/image9.jpg)
![Scaffold 3D detail](https://assemblingsugars.fr/wp-content/uploads/2020/06/image34.jpg)
![Confocal](https://assemblingsugars.fr/wp-content/uploads/2020/06/image10.jpg)
Neurons network (left) and neurosphere (right) of adult Human Neural Stem Cells (hNSC) (7 days, green immunofluorescent marker : Tuj1), after dissolution of the N-heptyl-D-galactonamide hydrogel.
Glial cells from adult Human Neural Stem Cells (hNSC) (7 days, green immunofluorescent marker : S100B), after dissolution of the N-heptyl-D-galactonamide hydrogel.